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Background: The ability to label and track biomolecules within living cells is crucial for understanding cellular processes and developing therapeutic strategies. While DNA-conjugated CuAAC (copper-catalyzed azide-alkyne cycloaddition) ligands offer powerful labeling capabilities, their delivery into cells remains challenging due to the large size and charge of DNA molecules. Current delivery methods often result in poor cellular uptake, uneven distribution, or cellular toxicity. The lack of efficient intracellular delivery systems for DNA-modified ligands limits their application in live cell imaging and biomolecular tracking studies.
Technical Overview: Northeastern researchers have developed an innovative method for encapsulating DNA-modified CuAAC ligands using liposomal formulations specifically designed for efficient intracellular delivery. The technology employs optimized lipid compositions and encapsulation protocols to protect the DNA-ligand conjugates during cellular uptake and ensure uniform distribution within cells. The system minimizes cellular toxicity while maintaining the functionality of the click chemistry reaction inside living cells. The delivery platform can be customized for different cell types and experimental conditions.
Benefits:
- Enables uniform intracellular delivery of DNA-modified CuAAC ligands
- Minimizes cellular toxicity compared to traditional delivery methods
- Maintains click chemistry functionality inside living cells
- Provides customizable delivery platform for various cell types
- Enhances the efficiency of biomolecular labeling in live cells
Application:
- Intracellular Biomolecule Labeling: Enhanced visualization and tracking of cellular components
- Live Cell Imaging: Real-time monitoring of cellular processes
- Drug Discovery: Studying intracellular drug distribution and interactions
- Cell Biology Research: Investigating cellular mechanisms and pathways
Opportunity:
- License
- Research collaboration
